Journal: Scientific Reports

Article Title: MiR-5095 inhibits proliferation, migration, and invasion of gastric cancer cells by targeting CEACAM5

doi: 10.1038/s41598-025-23669-6

Figure Lengend Snippet: Target gene screening for miR-5095. ( A ) Venn diagram showing the intersection of genes from the GEPIA2, GeneCards, and TargetScan databases. ( B ) The 24 genes regulated by miR-5095 identified through screening. ( C ) Protein-protein interaction network for CEACAM5 from STRING database. (D) Enrichment analysis for the top 26 proteins interacting with CEACAM5.

Article Snippet: Membranes were incubated overnight at 4 °C with primary antibodies against CEACAM5 (1:1000, Proteintech), E-cadherin (1:1000, Proteintech), N-cadherin (1:1000, Proteintech), and Vimentin (1:1000, Proteintech).

Techniques:

Journal: Scientific Reports

Article Title: MiR-5095 inhibits proliferation, migration, and invasion of gastric cancer cells by targeting CEACAM5

doi: 10.1038/s41598-025-23669-6

Figure Lengend Snippet: Validation of CEACAM5 as a target gene of miR-5095. ( A ) mRNA expression of CEACAM5 in tumor tissues ( n = 408) and normal tissues ( n = 211) from the GEPIA2 database. ( B ) Dual-luciferase reporter assay showing the binding sites and fluorescence ratio changes (ns: no significance). Statistical comparisons between groups were performed using unpaired two-tailed t-tests. CEACAM5-WT + miR-5095 mimic vs. CEACAM5-WT + NC mimic group ( P < 0.0001). CEACAM5-Mut + miR-5095 mimic vs. CEACAM5-Mut + NC mimic group ( P = 0.9598). ( C ) Western blotting assay detecting the expression of CEACAM5 in gastric cancer cells ( n = 3). Statistical analysis was performed using unpaired two-tailed t-tests comparing miR-5095 mimics to mimic NC: AGS cells ( P < 0.0001); HGC-27 cells ( P = 0.0002). Representative image shown from three independent experiments. ( D ) RT-qPCR measurement of CEACAM5 expression in gastric cancer cells. Statistical analysis was performed using unpaired two-tailed t-tests comparing miR-5095 mimics to mimic NC: AGS cells ( P < 0.0001); HGC-27 cells ( P = 0.0001). * P < 0.05, ** P < 0.01.

Article Snippet: Membranes were incubated overnight at 4 °C with primary antibodies against CEACAM5 (1:1000, Proteintech), E-cadherin (1:1000, Proteintech), N-cadherin (1:1000, Proteintech), and Vimentin (1:1000, Proteintech).

Techniques: Biomarker Discovery, Expressing, Luciferase, Reporter Assay, Binding Assay, Fluorescence, Two Tailed Test, Western Blot, Quantitative RT-PCR

Journal: Scientific Reports

Article Title: MiR-5095 inhibits proliferation, migration, and invasion of gastric cancer cells by targeting CEACAM5

doi: 10.1038/s41598-025-23669-6

Figure Lengend Snippet: miR-5095 targets CEACAM5 to inhibit gastric cancer cell proliferation, migration, and invasion. ( A ) Western blotting assay detecting CEACAM5 protein expression ( n = 3). OE-NC + miR-5095 mimics vs. OE-NC + NC mimics group (AGS: P = 0.0009, HGC-27: P < 0.0001, unpaired two-tailed t-test). OE-CEACAM5 + miR-5095 mimics group vs. OE-NC + miR-5095 mimics group (AGS: P < 0.0001, HGC-27: P < 0.0001, unpaired two-tailed t-test). Representative image shown from three independent experiments. ( B ) CCK-8 assay showing the change in cell viability ( n = 3). OE-NC + miR-5095 mimics vs. OE-NC + NC mimics group (AGS: P < 0.0001, HGC-27: P < 0.0001, unpaired two-tailed t-test). OE-CEACAM5 + miR-5095 mimics group vs. OE-NC + miR-5095 mimics group (AGS: P < 0.0013, HGC-27: P = 0.0008, unpaired two-tailed t-test). ( C ) Western blotting assay detecting the expression of EMT-related proteins ( n = 3). Statistical analysis was performed using multiple unpaired two-tailed t-tests. OE-NC + miR-5095 mimics vs. OE-NC + NC mimics group: AGS: E-cadherin ( P = 0.0003), N-cadherin ( P < 0.0001), Vimentin ( P = 0.0002); HGC-27: E-cadherin ( P = 0.0006), N-cadherin ( P = 0.0060), Vimentin ( P = 0.0017). OE-CEACAM5 + miR-5095 mimics group vs. OE-NC + miR-5095 mimics group: AGS: E-cadherin ( P = 0.0028), N-cadherin ( P < 0.0001), Vimentin ( P < 0.0001); HGC-27: E-cadherin ( P = 0.0313), N-cadherin ( P < 0.0091), Vimentin ( P = 0.0046). Representative image shown from three independent experiments. Transwell migration ( D ) and invasion ( E ) assay assessing the migration and invasion ability of gastric cancer cells ( n = 3, scale bar = 200 μm). * P < 0.05, ** P < 0.01.

Article Snippet: Membranes were incubated overnight at 4 °C with primary antibodies against CEACAM5 (1:1000, Proteintech), E-cadherin (1:1000, Proteintech), N-cadherin (1:1000, Proteintech), and Vimentin (1:1000, Proteintech).

Techniques: Migration, Western Blot, Expressing, Two Tailed Test, CCK-8 Assay

Journal: Journal of Translational Medicine

Article Title: A dynamic molecular landscape in colorectal cancer progression at single-cell resolution

doi: 10.1186/s12967-025-06785-9

Figure Lengend Snippet: Tumor-like epithelial cells identified in epithelial cells from CRC tissues. A The UMAP plot displaying the annotation of four sub-types of epithelial cells with corresponding color codes. B A UMAP plot illustrating the distribution of the four sub-cell types of epithelial cells across the four types of colorectal samples, with red arrows indicating specific cell types within the corresponding sample types. C InferCNV analyses shown in the violin plot indicate the genomic variation and malignancy across all cell types, with a red arrow highlighting the epithelial cells. Epithelial cells of the four sub-types ( D ) and from each sample type ( E ) are depicted in developmental trajectory analyses. Tumor-like epithelial cells from CRC tissues are highlighted in the red dashed square. Feature plots of BEST4 + ( F ) and CEACAM5 + ( G ) expression in epithelial cells are highlighted by the red arrows, respectively

Article Snippet: CEACAM5 , Anti-CEA (CD66e) CEACAM5 Rabbit Monoclonal Antibody , Boster , M00356-1 , 1:100.

Techniques: Expressing

Journal: Journal of Translational Medicine

Article Title: A dynamic molecular landscape in colorectal cancer progression at single-cell resolution

doi: 10.1186/s12967-025-06785-9

Figure Lengend Snippet: Immunofluorescence staining visualizes BEST4 + OTOP2 + and tumor-like epithelial cells in colorectal tissues. A Combined IF staining for CLDN4 (green channel, pan-marker gene for epithelial cells) and BEST4 (red channel) demonstrating the presence of BEST4 + epithelial cells in the four types of clinical colorectal samples, predominant in normal tissues. B Combined IF staining for CLDN4 (green channel) and CEACAM5 (red channel, pan-cancer cancer marker gene) showing the presence of CEACAM5 + epithelial cells, enriched in CRC tissure. Nuclei were counter-stained with DAPI. Tissues within the white dashed squares were imaged at higher magnification as displayed on the right side. Each staining was performed in triplicates using tissue slides sourced from three different individuals. A scale bar is present in each image

Article Snippet: CEACAM5 , Anti-CEA (CD66e) CEACAM5 Rabbit Monoclonal Antibody , Boster , M00356-1 , 1:100.

Techniques: Immunofluorescence, Staining, Marker